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髓清丸对HEL细胞系作用的血清药理学研究
引用本文:杨振江,郭岳峰,丘和明,张惠臣.髓清丸对HEL细胞系作用的血清药理学研究[J].中国中医基础医学杂志,2003,9(6):30-32,67.
作者姓名:杨振江  郭岳峰  丘和明  张惠臣
作者单位:1. 深圳市中医院,广东,深圳,518033
2. 河南省中医院,河南,郑州,450003
3. 广州中医药大学,广东,广州,510407
摘    要:目的 :研究中药复方髓清丸对白血病细胞的作用。方法 :采用血清药理学方法体外培养观察人红白血病细胞系 (HEL)的增殖、凋亡、细胞周期、细胞形态等细胞学指标。结果 :活细胞百分率各含药血清组均较对照组低 ,MTT法测定髓清丸中剂量组和高剂量组OD值明显低于对照组 (p<0 0 1) ,中剂量组和高剂量组细胞毒活性均大于 2 0 %,高剂量含药血清组G1期DNA含量明显低于对照组 (P <0 0 5 ) ,中剂量组凋亡细胞百分率较高 (P <0 0 5 )。结论 :髓清丸能抑制HEL细胞的增殖 ,并有一定的诱导HEL细胞分化、凋亡的作用。

关 键 词:髓清丸  HEL  血清药理学  白血病  细胞周期  细胞凋亡  细胞增殖
文章编号:1006-3250(2003)06-0030-04

Study on The Human Erythroleukemia Cell Line (HEL) with Serum-Pharmacology Methods
YANG Zhen-jiang,GUO Yue-feng,QIU He-ming,ZHANG Hui-chen.Study on The Human Erythroleukemia Cell Line (HEL) with Serum-Pharmacology Methods[J].Chinese Journal of Basic Medicine In Traditional Chinese Medicine,2003,9(6):30-32,67.
Authors:YANG Zhen-jiang  GUO Yue-feng  QIU He-ming  ZHANG Hui-chen
Abstract:To study the effect of Sui-Qing-Wan (SQW) on l ukemia cell.Methods: Human erythroleukemia cell line (HEL) was studied in Prolif eration,Apoptosis,cell proliferous cycle,Cytomorphology with serum-pharmacology methods.Results:The live cell percent,live cell absolute count and optical dens ity (OD) of MTT in three dose group were all lower than those in control group d istinctly ( P<0.05 ),cytotoxicity in the moderate group and the high dose gro up were all larger than 20%.DNA content of G 1 period in high dose group of SQW was higher than those in control group markedly ( P<0.05 ).It indicated that SQW could restrain malignant cell growth.Apoptosis peak was found in HEL cutured with YDQ moderate dose by FCM( P<0.05 ).Conclution:SQW can inhibite prolifer ation of HEL cell,induce differentiations and impell apoptosis.
Keywords:Sui-Qing-Wan(SQW)  Human erythroleukemia cell  line (HEL)  Proliferation  Apoptosis  
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