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胆酸类成分HPLC/ELSD单一标准品替代法含量测定研究
引用本文:叶磊,曹进,徐燕,王义明,罗国安.胆酸类成分HPLC/ELSD单一标准品替代法含量测定研究[J].中成药,2004,26(9):750-753.
作者姓名:叶磊  曹进  徐燕  王义明  罗国安
作者单位:清华大学分析中心,北京,100084
基金项目:国家重点基础研究发展计划(973计划)
摘    要:目的:对5种胆汁酸类成分的含量测定进行研究.方法:采用HPLC/ESLD方法,通过单一标准品替代法对5种胆汁酸成分进行定量测定,比较添加基质(兔血清、尿液和清开灵提取物)对测定结果的影响.结果:利用5种胆酸类成分中任一标准品就可以测定全部5种胆酸类成分的含量.结论:方法简单可靠,为复杂基质中成分的定量,特别是中药指纹图谱成分的量化控制提供了方法和研究思路.

关 键 词:胆汁酸  蒸发光散射检测  高效液相色谱  单一标准品替代
文章编号:1001-1528(2004)09-0750-04

HPLC/ELSD assay of cholic acid compounds by single standard substitute
YE Lei,CAO Jin,XU Yan,WANG Yi-ming,LUO Guo-an.HPLC/ELSD assay of cholic acid compounds by single standard substitute[J].Chinese Traditional Patent Medicine,2004,26(9):750-753.
Authors:YE Lei  CAO Jin  XU Yan  WANG Yi-ming  LUO Guo-an
Abstract:AIM: To study the determination of 5 kinds of cholic acid content. METHODS: Using single standard substitue (SSS), we assayed 5 kinds of cholic acid, such as cholic acid, deoxycholic acid, hyodexycholic acid, chenodeoxychoilc acid and ursodeoxycholic acid by HPLC/ELSD. and studied the influence of the additive substitutes (such as rabbit Serum, urine and Qingkailing extract) on the measure values. RESULTS: By using any one of the 5 kinds of cholic acid standard, the content in all the 5 kinds of cholic acid could be determined. CONCLUSION: Simply as well as credibly, the SSS method could be used in quality analysis, especially in complex matrix. Also, it proved a feasible way in quality control and research of fingerprint chromatography of TCM.
Keywords:bile acid  ELSD  HPLC  Single standard substitute
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