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海龟作为滋补品和药品已有悠久的历史。为了探索海龟对机体免疫作用的影响,本试验对海龟组织浆对肿瘤细胞的抑制和杀伤作用和抗衰老效果进行了研究,以期为海龟的全面开发利用提供理论依据。1材料与方法1.1试验材料绿海龟(Cheloniamydas)取自海南三亚,6周龄,体重751g,雄性。取腿部肌肉组织块用玻璃匀浆器匀浆,用蒸馏水稀释成浓度为50mg/ml的组织浆液,12000r/min离心15min,取上清液分装于10ml安培瓶中,置-20℃低温冰箱保存备用。体内试验:一级ICR小鼠100只,体重23±2g;一级wi… 相似文献
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以合成的开链聚醚三(二环己酰胺)等中性载体制成了PVC膜Li~+电极,研究了载体结构、膜成分与电极性能的关系,在聚醚三酰胺的胺基中,以环己基取代直链的烷基,显著地改善了Li~+—载体的性能,以邻苯二甲酸二癸酯或其和二苄醚混合增塑的1,1,1—三[1′—(2′—氧杂—3′—二环己胺基羰基)丙基]丙烷Li~+—电极的线性响应范围为2×10~(-5)~10~(-1)mol/L LiCl,K_(Li+Na)~(pot)~8×10~(-3),以Mg(Ac)_2为离子强度调节缓冲剂,用标准比较法或样品加入法测定了LiCl—HCl—H_2O或LiCl—MgCl_2—HCl—H_2O体系中的Li~+,LiCl含量≥2%的样品,测定误差≤5%。 相似文献
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设计了小流量流通池,制备了低阻锂离子选择电极,模拟在线监测核反应堆一回路含铵水质中的氢氧化锂,9:1体积比混合液样和PH5.5的Ax10^-2mol/LNH4Ac-HAc离子强度调节缓冲剂,泵入由聚醚三(二环己酰胺)PVC膜锂电极和Ag/AgCl参比电级构成的流通池,电位法测定,锂含量大于2×10^-5mol/L时,误差在5%以内。 相似文献
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羊栖菜(Sargassum fusiforme)多糖在多种肿瘤细胞系中表现出良好的抗癌活性,具有针对肿瘤细胞的选择活性和较小的毒副作用,可以作为现有肿瘤化疗药物的替代品进行开发。羊栖菜多糖主要通过诱导细胞凋亡作用于肿瘤细胞,其通过细胞周期停滞,增强机体免疫功能,改变细胞膜钙通道与流动性,破坏线粒体膜和产生一氧化氮来杀死癌细胞并防止转移。本文就国内外发表的羊栖菜多糖抗肿瘤功能及作用机制进行系统的归纳和总结,旨在为羊栖菜多糖抗肿瘤药物的深入研究、开发提供理论依据。 相似文献
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Nitrogen balance method and nitrogen-free diet were used in this study to determine nitrogen maintenance requirement (NM)
and nitrogen maintenance requirement per unit metabolism body weight (NM′) of black porgy Acanthopagrus schlegeli. Fish with body weight (BW) of 50, 80, 120, 160 and 200 g were fed by the diets containing three graded levels of crude protein
(380, 420 and 460 g/kg). The results from nitrogen balance experiment showed that the amount of nitrogen deposition varied
from 0.15 to 0.31 mg/g BW per day, accounting for 12.2% to 21.1% of nitrogen intake. The amount of fecal nitrogen excretion
varied from 0.21 to 0.32 mg/g BW per day, accounting for 16.3% to 21.6% of nitrogen intake. The endogenous nitrogen excretion,
a main part of nitrogen consumption varied from 0.79 to 0.97 mg/g BW per day, accounting for 63.3 % to 68.0% of nitrogen intake.
Positive correlation was found between NM and body weight, while a negative correlation was found between NM of unit body
weight and the growth duration. No significant differences (p>0.05) were found in NM′ among different growth stages. The average of NM′ was 0.485 7mg/g per day. The results from nitrogen-free
diet experiment showed that a negative correlation between NM and feed intake of nitrogen-free diet. NM increased with the
decrease of feed intake of fish. The average of NM was 0.482 9 mg/g BW per day that was close to 0.483 8 mg/g BW obtained
from fish with 120 g BW in nitrogen balance experiment. The nitrogen balance method is recommended to be a better method for
determining NM in consideration of fish stress and result stability. This study also provides a calculated result of the protein
content in diets, which is necessary for maintaining fish body protein at different growth stages. The calculation was based
on the amount of nitrogen required for maintaining body protein per kg BW.
Supported by Scientific Research Project Grant (No.2004C100059) from the city government of Ningbo, China. 相似文献