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海龟作为滋补品和药品已有悠久的历史。为了探索海龟对机体免疫作用的影响,本试验对海龟组织浆对肿瘤细胞的抑制和杀伤作用和抗衰老效果进行了研究,以期为海龟的全面开发利用提供理论依据。1材料与方法1.1试验材料绿海龟(Cheloniamydas)取自海南三亚,6周龄,体重751g,雄性。取腿部肌肉组织块用玻璃匀浆器匀浆,用蒸馏水稀释成浓度为50mg/ml的组织浆液,12000r/min离心15min,取上清液分装于10ml安培瓶中,置-20℃低温冰箱保存备用。体内试验:一级ICR小鼠100只,体重23±2g;一级wi…  相似文献   
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Nitrogen balance method and nitrogen-free diet were used in this study to determine nitrogen maintenance requirement (NM) and nitrogen maintenance requirement per unit metabolism body weight (NM′) of black porgy Acanthopagrus schlegeli. Fish with body weight (BW) of 50, 80, 120, 160 and 200 g were fed by the diets containing three graded levels of crude protein (380, 420 and 460 g/kg). The results from nitrogen balance experiment showed that the amount of nitrogen deposition varied from 0.15 to 0.31 mg/g BW per day, accounting for 12.2% to 21.1% of nitrogen intake. The amount of fecal nitrogen excretion varied from 0.21 to 0.32 mg/g BW per day, accounting for 16.3% to 21.6% of nitrogen intake. The endogenous nitrogen excretion, a main part of nitrogen consumption varied from 0.79 to 0.97 mg/g BW per day, accounting for 63.3 % to 68.0% of nitrogen intake. Positive correlation was found between NM and body weight, while a negative correlation was found between NM of unit body weight and the growth duration. No significant differences (p>0.05) were found in NM′ among different growth stages. The average of NM′ was 0.485 7mg/g per day. The results from nitrogen-free diet experiment showed that a negative correlation between NM and feed intake of nitrogen-free diet. NM increased with the decrease of feed intake of fish. The average of NM was 0.482 9 mg/g BW per day that was close to 0.483 8 mg/g BW obtained from fish with 120 g BW in nitrogen balance experiment. The nitrogen balance method is recommended to be a better method for determining NM in consideration of fish stress and result stability. This study also provides a calculated result of the protein content in diets, which is necessary for maintaining fish body protein at different growth stages. The calculation was based on the amount of nitrogen required for maintaining body protein per kg BW. Supported by Scientific Research Project Grant (No.2004C100059) from the city government of Ningbo, China.  相似文献   
3.
Nitrogen balance method and nitrogen-free diet were used in this study to determine nitrogen maintenance requirement (NM) and nitrogen maintenance requirement per unit metabolism body weight (NM’) of black porgy Acanthopagrus schlegeli. Fish with body weight (BW) of 50, 80, 120, 160 and 200 g were fed by the diets containing three graded levels of crude protein (380, 420 and 460 g/kg). The results from nitrogen balance experiment showed that the amount of nitrogen deposition varied from 0.15 to 0.31 mg/g BW...  相似文献   
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日本沼虾染色体倍性检测技术的研究   总被引:2,自引:0,他引:2  
用流式细胞仪分别对日本沼虾(Macrobrachiumnipponense)正常二倍体的体细胞和四倍阶段体胚胎的DNA相对含量和细胞频率进行了测定,以研究日本沼虾染色体倍性检测技术。以日本沼虾正常二倍体的体细胞液进样所得直方图为标准,用对细胞松弛素B人工诱导的日本沼虾四倍体胚胎进行DNA相对含量和细胞频率的测定,测试结果以直方图表示,并用染色体涂片镜检结果进行倍性核对。结果表明,日本沼虾二倍体和四倍体的DNA相对含量有显著差异,四倍体日本沼虾的DNA相对含量是二倍体的2倍。用流式细胞仪法检测日本沼虾倍性,方法简单、测试速度快、结果可靠。  相似文献   
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